Anti-Superoxide Dismutase 1 antibody (ab22740)

Overview

  • Product nameAnti-Superoxide Dismutase 1 antibody
    See all Superoxide Dismutase 1 primary antibodies
  • Description
    Rabbit polyclonal to Superoxide Dismutase 1
  • SpecificityWestern blot shows high specificity to mouse SOD1. This antibody recognizes mouse SOD-1, but does not recognize human SOD-1. It is therefore useful for the examination of mice carrying the transgenic human SOD-1 gene.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Human
  • Immunogen

    Synthetic peptide:

    H-ASGEPVVLSGQIT-NH2

    , corresponding to amino acids 25-37 of Mouse Superoxide Dismutase 1 conjugated to diphtheria toxoid. A cysteine was added to the C terminus for conjugation.

Properties

Applications

Our Abpromise guarantee covers the use of ab22740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 16 kDa.

Target

  • FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    modifications
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

References for Anti-Superoxide Dismutase 1 antibody (ab22740)

ab22740 has not yet been referenced specifically in any publications.

Product Wall

I am very pleased to hear you would like to accept our offer and test ab22740 in IHC-Fr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the...

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Thank you for your enquiry. I can confirm that the WB conditions used here are: - blocking with 5% skim milk powder in PBS containing 0.1%Tween-20 - overnight incubation - primary dilution used was 1:100 dilution, but has also been shown t...

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