Overview

  • Product name
    Anti-Superoxide Dismutase 3 antibody [1H12]
    See all Superoxide Dismutase 3 primary antibodies
  • Description
    Mouse monoclonal [1H12] to Superoxide Dismutase 3
  • Tested applications
    Suitable for: WB, IP, IHC-P, ChIP, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) purified from E.coli.

  • Positive control
    • Bosc23 cell lysate transfected with Myc-His-SOD3.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR58884 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab28442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa.
IP Use at an assay dependent concentration.

Use 2μg

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • Tissue specificity
    Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • Sequence similarities
    Belongs to the Cu-Zn superoxide dismutase family.
  • Cellular localization
    Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Images

  • All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)

    Lane 1 : Bosc23 cell lysate
    Lane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3
    Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3


    Predicted band size : 26 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab28442 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Nightingale H  et al. Changes in Expression of the Antioxidant Enzyme SOD3 Occur Upon Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells In Vitro. Stem Cells Dev : (2012). Read more (PubMed: 22132904) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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