Anti-Superoxide Dismutase 3 antibody [4G11G6] (ab80946)

Overview

  • Product nameAnti-Superoxide Dismutase 3 antibody [4G11G6]
    See all Superoxide Dismutase 3 primary antibodies
  • Description
    Mouse monoclonal [4G11G6] to Superoxide Dismutase 3
  • SpecificityDetects extracellular Superoxide Dismutase 3
  • Tested applicationsSuitable for: Flow Cyt, ELISA, ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native Human protein purified from aortas

  • Positive control
    • Human cartilage lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionProtect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • Tissue specificityExpressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
  • Cellular localizationSecreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Anti-Superoxide Dismutase 3 antibody [4G11G6] images

  • ab80946 at 1/100 dilution staining Superoxide Dismutase 3 in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis. 

  • Anti-Superoxide Dismutase 3 antibody [4G11G6] (ab80946) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Overlay histogram showing HEK293 cells stained with ab80946 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80946, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-Superoxide Dismutase 3 antibody [4G11G6] (ab80946)

This product has been referenced in:
  • Arcucci A  et al. Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve. Eur J Histochem 58:2383 (2014). WB ; Human . Read more (PubMed: 25308842) »
  • Regan E  et al. Extracellular superoxide dismutase and oxidant damage in osteoarthritis. Arthritis Rheum 52:3479-91 (2005). ELISA ; Human . Read more (PubMed: 16255039) »

See all 2 Publications for this product

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My colleague Elena has been out of the office, so I've sent your image on to the production laboratory and also requested the testing data from them. I a...

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I am sorry you are experiencing difficulty with this product. Since you obtained good results with the pulmonary artery tissue, it seems that maybe the isolation process is causing some extra bands. Can you give ...

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I am sorry that this antibody did not perform as stated on the datasheet. The amount paid for the antibody will be refunded to your credit card. To avoid confusion, please ensure your accounts department is aware...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"