• Product nameAnti-Superoxide Dismutase 3 antibody
    See all Superoxide Dismutase 3 primary antibodies
  • Description
    Rabbit polyclonal to Superoxide Dismutase 3
  • SpecificitySpecific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 19-37 of Human Superoxide Dismutase 3.

  • Positive control
    • IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate



Our Abpromise guarantee covers the use of ab21974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/2000.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).


  • FunctionProtect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • Tissue specificityExpressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
  • Cellular localizationSecreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Anti-Superoxide Dismutase 3 antibody images

  • ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
  • ab21974 (1/1000) staining of human kidney tubule cells.
  • All lanes : Anti-Superoxide Dismutase 3 antibody (ab21974) at 1/500 dilution

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-Superoxide Dismutase 3 antibody (ab21974)

This product has been referenced in:
  • Duscher D  et al. Aging disrupts cell subpopulation dynamics and diminishes the function of mesenchymal stem cells. Sci Rep 4:7144 (2014). IHC-Fr ; Mouse . Read more (PubMed: 25413454) »
  • Cunningham MW  et al. Renal nitric oxide synthase and antioxidant preservation in cyp1a1-ren-2 transgenic rats with inducible malignant hypertension. Am J Hypertens 26:1242-9 (2013). Read more (PubMed: 23764378) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Citrate Buffer + 0.05% Tween 20
Specification Lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Formaldehyde

Dr. I Zelko

Verified customer

Submitted Jan 25 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse Penile Tissue)
Loading amount 30 µg
Specification Mouse Penile Tissue
Gel Running Conditions Reduced Denaturing (4-15% minigel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. gwen Lagoda

Verified customer

Submitted Jan 25 2011