Anti-Superoxide Dismutase 3 antibody (ab90258)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab90258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).

Target

  • FunctionProtect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • Tissue specificityExpressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
  • Cellular localizationSecreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Anti-Superoxide Dismutase 3 antibody images

  • All lanes : Anti-Superoxide Dismutase 3 antibody (ab90258) at 1 µg/ml

    Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 32,35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,42 kDa,50 kDa,55 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-Superoxide Dismutase 3 antibody (ab90258)

ab90258 has not yet been referenced specifically in any publications.

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