Anti-Superoxide Dismutase 4 antibody [2A1] (ab16963)

Overview

  • Product nameAnti-Superoxide Dismutase 4 antibody [2A1]
    See all Superoxide Dismutase 4 primary antibodies
  • Description
    Mouse monoclonal [2A1] to Superoxide Dismutase 4
  • Tested applicationsSuitable for: ICC/IF, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab16963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 1 - 2 µg/ml.

Target

  • FunctionDelivers copper to copper zinc superoxide dismutase (SOD1).
  • Tissue specificityUbiquitous.
  • Sequence similaritiesIn the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.
    Contains 1 HMA domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCS antibody
    • CCS_HUMAN antibody
    • Copper chaperone for superoxide dismutase antibody
    • MGC138260 antibody
    • SOD 4 antibody
    • SOD4 antibody
    • Superoxide dismutase copper chaperone antibody
    see all

Anti-Superoxide Dismutase 4 antibody [2A1] images

  • ab16963 staining Superoxide Dismutse 4 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • Immunoprecipitation analysis of HeLa cell lysates using ab16963.
    Lane 1- Input
    Lane 2- IP

    Western analysis was conducted using a polyclonal SOD4 antibody (ab16834)

References for Anti-Superoxide Dismutase 4 antibody [2A1] (ab16963)

ab16963 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"