Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
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Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.
|SOD Assay Buffer||WM||1 x 20ml|
|SOD Dilution Buffer||NM||1 x 10ml|
|SOD Enzyme Solution||Green||1 x 20µl|
|WST Solution||Red||1 x 1ml|
Our Abpromise guarantee covers the use of ab65354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).
* Indicates statistical significance in comparison with DM group (P < 0.05). # Indicates statistical significance in comparison with the statin group (P<0.05).
Superoxide dismutase measured in biofluids at various dilutions
Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)