Anti-Suppressor of Fused antibody (ab28083)

Overview

  • Product nameAnti-Suppressor of Fused antibody
    See all Suppressor of Fused primary antibodies
  • Description
    Rabbit polyclonal to Suppressor of Fused
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Suppressor of Fused.

    (Peptide available as ab28933.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) This antibody gave a positive signal in the following tissue lysate: Testis (Mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab28083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • FunctionNegative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24311597, PubMed:24217340). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24311597, PubMed:24217340). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation.
  • Tissue specificityUbiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney.
  • Involvement in diseaseMedulloblastoma
  • Sequence similaritiesBelongs to the SUFU family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000020374 antibody
    • OTTHUMP00000020377 antibody
    • OTTHUMP00000020379 antibody
    • PRO1280 antibody
    • SU FU antibody
    • SU(F)U antibody
    • Su(fu) antibody
    • SUFU antibody
    • SUFU negative regulator of hedgehog signaling antibody
    • SUFU_HUMAN antibody
    • SUFUH antibody
    • SUFUXL antibody
    • Suppressor of fused homolog (Drosophila) antibody
    • Suppressor of fused homolog antibody
    see all

Anti-Suppressor of Fused antibody images



  • Predicted band size : 54 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Suppressor of Fused knockout HAP1 cell lysate (20 µg)
    Lane 3: LnCaP cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 to 4: Merged signal (red and green). Green - ab28083 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab28083 was shown to recognize Suppressor of Fused when Suppressor of Fused knockout samples were used, along with additional cross-reactive bands. Wild-type and Suppressor of Fused knockout samples were subjected to SDS-PAGE. ab28083 and ab8245 (loading control to GAPDH) were both diluted at 1 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Suppressor of Fused antibody (ab28083) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 37 kDa (possible cleavage fragment,cross reactivity).
  • Anti-Suppressor of Fused antibody (ab28083) at 1 µg/ml + Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ICC/IF image of ab28083 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28083, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-Suppressor of Fused antibody (ab28083)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Mar 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293, SKOV-3, CaOV-3)
Loading amount 15 µg
Specification HEK293, SKOV-3, CaOV-3
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Aqua Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2011

Thank you for your enquiry. This antibody has only been tested in Western Blot. That does not mean that it won't work in IHC applications, it just means that we haven't tested it and therefore can not guarantee that it will work in those applications. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"