Anti-Suppressor of Ty 4 homolog 1 antibody [3079C1a] (ab54350)

Overview

  • Product nameAnti-Suppressor of Ty 4 homolog 1 antibody [3079C1a]
    See all Suppressor of Ty 4 homolog 1 primary antibodies
  • Description
    Mouse monoclonal [3079C1a] to Suppressor of Ty 4 homolog 1
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human) from an internal sequence.

Properties

Applications

Our Abpromise guarantee covers the use of ab54350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionComponent of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the SPT4 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DRB sensitivity-inducing factor 14 kDa subunit antibody
    • DRB sensitivity-inducing factor small subunit antibody
    • DSIF p14 antibody
    • DSIF small subunit antibody
    • hSPT4 antibody
    • SPT4 antibody
    • SPT4H antibody
    • SPT4H_HUMAN antibody
    • SUPT4H `1 antibody
    • SUPT4H antibody
    • Supt4h1 antibody
    • Transcription elongation factor spt4 antibody
    see all

Anti-Suppressor of Ty 4 homolog 1 antibody [3079C1a] images

  • Anti-Suppressor of Ty 4 homolog 1 antibody [3079C1a] (ab54350) at 1/100 dilution + immunising recombinant protein

    Secondary
    Sheep Anti Mouse IgG at 1/3000 dilution

    Predicted band size : 13 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-Suppressor of Ty 4 homolog 1 antibody [3079C1a] (ab54350)

ab54350 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés.

La bande obtenue avec le ab54530 correspond à la protéine ayant servi comme immunogène (13 kDa)et non à la protéine endogène (32 kDa).
http://www.abcam.com/sup...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"