Anti-SUPT4H antibody [3079C1a] (ab54350)
Key features and details
- Mouse monoclonal [3079C1a] to SUPT4H
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-SUPT4H antibody [3079C1a]
See all SUPT4H primary antibodies -
Description
Mouse monoclonal [3079C1a] to SUPT4H -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human SUPT4H (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
3079C1a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
Target
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Function
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the SPT4 family. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DRB sensitivity-inducing factor 14 kDa subunit antibody
- DRB sensitivity-inducing factor small subunit antibody
- DSIF p14 antibody
see all
Images
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Anti-SUPT4H antibody [3079C1a] (ab54350) at 1/100 dilution + immunising recombinant protein
Secondary
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size: 13 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?The immunogen peptide in this image was tagged. Secondary antibody: Sheep Anti Mouse IgG (Original Conc.; Antibody:0.63mg/ml, HRP: 0.78mg/ml) Immunogen amount: 25ng/lane. MW of Tag: approx. 20kDa.
Antibody only tested on recombinant fragment as immunogen. We have no data on detection of endogenous protein.
Datasheets and documents
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Datasheet download
References (1)
ab54350 has been referenced in 1 publication.
- Liu CR et al. Spt4 is selectively required for transcription of extended trinucleotide repeats. Cell 148:690-701 (2012). PubMed: 22341442