Anti-SUPT16H antibody (ab204343)
Key features and details
- Rabbit polyclonal to SUPT16H
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SUPT16H antibody
See all SUPT16H primary antibodies -
Description
Rabbit polyclonal to SUPT16H -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis -
Immunogen
Recombinant fragment corresponding to Human SUPT16H aa 150-300.
Database link: Q9Y5B9 -
Positive control
- Human rectum tissue; RT-4 and U-251 MG cell lysates, Human plasma and Human tonsil tissue lysates; U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 120 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 120 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase M24 family. SPT16 subfamily. -
Domain
The Glu-rich acidic region in C-terminus is essential for FACT activity. -
Post-translational
modificationsADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin. -
Cellular localization
Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci. - Information by UniProt
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Database links
- Entrez Gene: 11198 Human
- Entrez Gene: 114741 Mouse
- Entrez Gene: 305851 Rat
- Entrez Gene: 399345 Xenopus laevis
- Omim: 605012 Human
- SwissProt: Q9Y5B9 Human
- SwissProt: Q920B9 Mouse
- SwissProt: Q9W603 Xenopus laevis
see all -
Alternative names
- CDC68 antibody
- Chromatin specific transcription elongation factor 140 kDa subunit antibody
- Chromatin-specific transcription elongation factor 140 kDa subunit antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling SUPT16H with ab204343 at 1/1000 dilution.
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All lanes : Anti-SUPT16H antibody (ab204343) at 1/500 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 120 kDa -
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling SUPT16H with ab204343 at 4 µg/ml (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab204343 has been referenced in 3 publications.
- Haase J et al. The TFIIH complex is required to establish and maintain mitotic chromosome structure. Elife 11:N/A (2022). PubMed: 35293859
- Song H et al. Targeting Histone Chaperone FACT Complex Overcomes 5-Fluorouracil Resistance in Colon Cancer. Mol Cancer Ther 19:258-269 (2020). PubMed: 31575655
- Porter LF et al. A role for repressive complexes and H3K9 di-methylation in PRDM5-associated brittle cornea syndrome. Hum Mol Genet 24:6565-79 (2015). IHC-P ; Human . PubMed: 26395458