Overview

  • Product nameAnti-SUPT16H antibody
    See all SUPT16H primary antibodies
  • Description
    Mouse monoclonal to SUPT16H
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 608-716 of Human SUPT16H

Properties

Applications

Our Abpromise guarantee covers the use of ab56855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the peptidase M24 family. SPT16 subfamily.
    • DomainThe Glu-rich acidic region in C-terminus is essential for FACT activity.
    • Post-translational
      modifications
      ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
    • Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC68 antibody
      • Chromatin specific transcription elongation factor 140 kDa subunit antibody
      • Chromatin-specific transcription elongation factor 140 kDa subunit antibody
      • Facilitates chromatin transcription complex subunit SPT16 antibody
      • FACT 140 kDa subunit antibody
      • FACT antibody
      • FACT complex subunit SPT16 antibody
      • FACTp140 antibody
      • FLJ10857 antibody
      • FLJ14010 antibody
      • hSPT16 antibody
      • SP16H_HUMAN antibody
      • Suppressor of Ty 16 homolog antibody
      • Supt16h antibody
      see all

    Anti-SUPT16H antibody images



    • Predicted band size : 120 kDa
      SUPT16H antibody (ab56855) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-SUPT16H antibody (ab56855)

    ab56855 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Loading amount 100 µg
    Specification HEK293 cells
    Gel Running Conditions Non-reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Sep 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"