• Product nameAnti-SUPT16H antibody
    See all SUPT16H primary antibodies
  • Description
    Rabbit polyclonal to SUPT16H
  • SpecificityDetects endogenous levels of total SUPT16H protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive control
    • HepG2 cell extract



Our Abpromise guarantee covers the use of ab64961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately >117 kDa (predicted molecular weight: 120 kDa).
ELISA 1/10000.


  • FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the peptidase M24 family. SPT16 subfamily.
  • DomainThe Glu-rich acidic region in C-terminus is essential for FACT activity.
  • Post-translational
    ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
  • Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC68 antibody
    • Chromatin specific transcription elongation factor 140 kDa subunit antibody
    • Chromatin-specific transcription elongation factor 140 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SPT16 antibody
    • FACT 140 kDa subunit antibody
    • FACT antibody
    • FACT complex subunit SPT16 antibody
    • FACTp140 antibody
    • FLJ10857 antibody
    • FLJ14010 antibody
    • hSPT16 antibody
    • SP16H_HUMAN antibody
    • Suppressor of Ty 16 homolog antibody
    • Supt16h antibody
    see all

Anti-SUPT16H antibody images

  • All lanes : Anti-SUPT16H antibody (ab64961) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 120 kDa
    Observed band size : >117 kDa (why is the actual band size different from the predicted?)

References for Anti-SUPT16H antibody (ab64961)

ab64961 has not yet been referenced specifically in any publications.

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