• Product nameAnti-SUPT5H antibody
    See all SUPT5H primary antibodies
  • Description
    Rabbit polyclonal to SUPT5H
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 396 - 445 (FEDQPEGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG) of Human SUPT5H (NP_003160)

  • Positive control
    • Jurkat cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab83974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionComponent of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SPT5 family.
    Contains 5 KOW domains.
  • Post-translational
    Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
    Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DRB sensitivity inducing factor large subunit antibody
    • DRB sensitivity-inducing factor 160 kDa subunit antibody
    • DRB sensitivity-inducing factor large subunit antibody
    • DSIF large subunit antibody
    • DSIF p160 antibody
    • FLJ34157 antibody
    • hSPT 5 antibody
    • hSPT5 antibody
    • SPT 5 antibody
    • SPT 5H antibody
    • SPT5 antibody
    • SPT5H antibody
    • SPT5H_HUMAN antibody
    • Suppressor of Ty 5 homolog antibody
    • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
    • SUPT 5H antibody
    • supt5h antibody
    • Tat cotransactivator 1 protein antibody
    • Tat CT1 antibody
    • Tat CT1 protein antibody
    • Tat-cotransactivator 1 protein antibody
    • Tat-CT1 protein antibody
    • Transcription elongation factor SPT 5 antibody
    • Transcription elongation factor spt5 antibody
    • Transcription factor Tat CT1 antibody
    see all

Anti-SUPT5H antibody images

  • Anti-SUPT5H antibody (ab83974) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 121 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SUPT5H antibody (ab83974)

ab83974 has not yet been referenced specifically in any publications.

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