• Product name
  • Description
    Mouse monoclonal to SUPT5H
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 981-1087 of Human SUPT5H (AAH24203) with a proprietary tag.

  • Positive control
    • Partial tagged recombinant Human SUPT5H protein (Immunogen), HeLa nuclear extract and HeLa cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab89219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 121 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SPT5 family.
    Contains 5 KOW domains.
  • Post-translational
    Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
    Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DRB sensitivity inducing factor large subunit antibody
    • DRB sensitivity-inducing factor 160 kDa subunit antibody
    • DRB sensitivity-inducing factor large subunit antibody
    • DSIF large subunit antibody
    • DSIF p160 antibody
    • FLJ34157 antibody
    • hSPT 5 antibody
    • hSPT5 antibody
    • SPT 5 antibody
    • SPT 5H antibody
    • SPT5 antibody
    • SPT5H antibody
    • SPT5H_HUMAN antibody
    • Suppressor of Ty 5 homolog antibody
    • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
    • SUPT 5H antibody
    • supt5h antibody
    • Tat cotransactivator 1 protein antibody
    • Tat CT1 antibody
    • Tat CT1 protein antibody
    • Tat-cotransactivator 1 protein antibody
    • Tat-CT1 protein antibody
    • Transcription elongation factor SPT 5 antibody
    • Transcription elongation factor spt5 antibody
    • Transcription factor Tat CT1 antibody
    see all


  • Anti-SUPT5H antibody (ab89219) at 5 µg/ml + Partial tagged recombinant Human SUPT5H protein at 0.2 µg

    Predicted band size : 121 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-SUPT5H antibody (ab89219) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • ab89219, at 10 µg/ml, staining SUPT5H in HeLa cells by Immunofluorescence.


ab89219 has not yet been referenced specifically in any publications.

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