Overview

  • Product name
    Anti-SUPT5H antibody [EPR5145(2)]
    See all SUPT5H primary antibodies
  • Description
    Rabbit monoclonal [EPR5145(2)] to SUPT5H
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SUPT5H.

  • Positive control
    • HeLa, HepG2, RAW264.7, PC12, NIH3T3, Jurkat and 293T cell lysates; Human colon tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 5.31 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5145(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 121 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the SPT5 family.
      Contains 5 KOW domains.
    • Post-translational
      modifications
      Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
      Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DRB sensitivity inducing factor large subunit antibody
      • DRB sensitivity-inducing factor 160 kDa subunit antibody
      • DRB sensitivity-inducing factor large subunit antibody
      • DSIF large subunit antibody
      • DSIF p160 antibody
      • FLJ34157 antibody
      • hSPT 5 antibody
      • hSPT5 antibody
      • SPT 5 antibody
      • SPT 5H antibody
      • SPT5 antibody
      • SPT5H antibody
      • SPT5H_HUMAN antibody
      • Suppressor of Ty 5 homolog antibody
      • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
      • SUPT 5H antibody
      • supt5h antibody
      • Tat cotransactivator 1 protein antibody
      • Tat CT1 antibody
      • Tat CT1 protein antibody
      • Tat-cotransactivator 1 protein antibody
      • Tat-CT1 protein antibody
      • Transcription elongation factor SPT 5 antibody
      • Transcription elongation factor spt5 antibody
      • Transcription factor Tat CT1 antibody
      see all

    Images

    • All lanes : Anti-SUPT5H antibody [EPR5145(2)] (ab126592) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : RAW264.7 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH 3T3 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 121 kDa
    • ab126592 at 1/100 dilution staining SUPT5H in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab126592 at 1/100 dilution staining SUPT5H in HeLa cells by immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Bolt MJ  et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24441042) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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