Overview

  • Product nameAnti-Surb7 antibody
    See all Surb7 primary antibodies
  • Description
    Mouse monoclonal to Surb7
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-145 of Human Surb7

Properties

Applications

Our Abpromise guarantee covers the use of ab56503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similaritiesBelongs to the Mediator complex subunit 21 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MED21 antibody
    • hSrb7 antibody
    • MED21 antibody
    • MED21_HUMAN antibody
    • Mediator complex subunit 21 antibody
    • Mediator of RNA polymerase II transcription subunit 21 antibody
    • RNA polymerase II holoenzyme component SRB7 antibody
    • RNAPII complex component SRB7 antibody
    • SRB7 antibody
    • SRB7 suppressor of RNA polymerase B homolog antibody
    • Surb7 antibody
    see all

Anti-Surb7 antibody images

  • Overlay histogram showing HeLa cells stained with ab56503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56503, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Surb7 antibody (ab56503) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-Surb7 antibody (ab56503)

ab56503 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"