The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 21 family.
Mediator of RNA polymerase II transcription subunit 21 antibody
RNA polymerase II holoenzyme component SRB7 antibody
RNAPII complex component SRB7 antibody
SRB7 suppressor of RNA polymerase B homolog antibody
Anti-Surb7 antibody - ChIP Grade images
ChIP - Anti-Surb7 antibody (ab123052)
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Western blot - Anti-Surb7 antibody (ab123052)
Anti-Surb7 antibody - ChIP Grade (ab123052) at 1 µg/ml + Mouse liver lysate at 10 µg
Predicted band size : 15 kDa Gel concentration: 10-20%
References for Anti-Surb7 antibody - ChIP Grade (ab123052)
has not yet been referenced specifically in any publications.
Publishing research using ab123052? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"