Anti-Surb7 antibody - ChIP Grade (ab123052)

Overview

  • Product nameAnti-Surb7 antibody - ChIP Grade
    See all Surb7 primary antibodies
  • Description
    Rabbit polyclonal to Surb7 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 19-68 (FCNAIGVLQQ CGPPASFSNI QTAINKDQPA NPTEEYAQLF AALIARTAKD) of Mouse Surb7 (NP_079591).

  • Positive control
    • Mouse liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab123052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similaritiesBelongs to the Mediator complex subunit 21 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MED21 antibody
    • hSrb7 antibody
    • MED21 antibody
    • MED21_HUMAN antibody
    • Mediator complex subunit 21 antibody
    • Mediator of RNA polymerase II transcription subunit 21 antibody
    • RNA polymerase II holoenzyme component SRB7 antibody
    • RNAPII complex component SRB7 antibody
    • SRB7 antibody
    • SRB7 suppressor of RNA polymerase B homolog antibody
    • Surb7 antibody
    see all

Anti-Surb7 antibody - ChIP Grade images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-Surb7 antibody - ChIP Grade (ab123052) at 1 µg/ml + Mouse liver lysate at 10 µg

    Predicted band size : 15 kDa
    Gel concentration: 10-20%

References for Anti-Surb7 antibody - ChIP Grade (ab123052)

ab123052 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"