Overview

  • Product name
  • Description
    Rabbit polyclonal to SURF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SURF2 aa 175-225. The exact sequence is proprietary.
    Sequence:

    LFTRKD LGSTEDGDGT DDFLTDKEDE KAKPPREKAT DESRRETTVY RGLVQ


    Database link: Q15527

  • Positive control
    • HeLa, 293T, and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-SURF2 antibody (ab195330) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 30 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation. ab195330 at 1 µg/ml staining SURF2 in 293T whole cell lysate immunoprecipitated using ab195330 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: control IgG.

References

ab195330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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