Overview

  • Product nameAnti-SURF5 antibody
    See all SURF5 primary antibodies
  • Description
    Mouse monoclonal to SURF5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
  • Immunogen

    Recombinant fragment: MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ , corresponding to amino acids 1-100 of Human SURF5 with a 26 kDa enzyme tag. (Swiss-Prot Q15528).

  • Positive control
    • Recombinant fragment of human SURF5.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 12 kDa plus a 26 kDa tag).
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-SURF5 antibody images

    • Anti-SURF5 antibody (ab76634) at 5 µg/ml + Recombinant fragment of human SURF5 with a 26 kDa tag, at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 12 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-SURF5 antibody (ab76634)

    ab76634 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76634.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"