Overview

  • Product nameAnti-Surf6 antibody
    See all Surf6 primary antibodies
  • Description
    Rabbit polyclonal to Surf6
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    A region within synthetic peptide: VEATPEGACT EPREPPGLIF NKVEVSEDEP ASKAQRRKEK RQRVKGNLTP, corresponding to internal sequence amino acids 181-230 of Human Surf6

  • Positive control
    • Jurkat cell lysates Human kidney tiussue Human heart tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-Surf6 antibody images

  • Lane 1 : Marker
    Lane 2 : Anti-Surf6 antibody (ab50926) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • Epithelial cells of human kidney renal tubule stained with ab50926 (arrows) at a concentration of 4-8 µg/ml. Magnification 400x
  • Myocardial cells of human heart stained with ab50926 (arrows) at a concentration of 4-8 µg/ml. Magnification 400x

References for Anti-Surf6 antibody (ab50926)

ab50926 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"