Overview

  • Product name
    Anti-Surfactant Protein A antibody [6F10]
    See all Surfactant Protein A primary antibodies
  • Description
    Mouse monoclonal [6F10] to Surfactant Protein A
  • Specificity
    ab51891 is specific for human pulmonary Surfactant Protein A
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Native Surfactant Protein A purified from bronchioaveolar lavage from proteinosis patients.

  • Positive control
    • Human lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab51891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21421565
WB 1/1000. Predicted molecular weight: 26 kDa.
ELISA 1/16000.
IHC-P Use at an assay dependent concentration.

Target

  • Cellular localization
    Secreted protein; extracellular space.
  • Database links
  • Alternative names
    • 35 kDa pulmonary surfactant-associated protein antibody
    • Alveolar proteinosis protein antibody
    • COLEC4 antibody
    • Collectin 4 antibody
    • PSAP antibody
    • PSPA antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant-associated protein A1 antibody
    • SFTP1 antibody
    • SFTPA antibody
    • SFTPA1 antibody
    • SFTPA1B antibody
    • SP A antibody
    • SP A1 antibody
    see all

Anti-Surfactant Protein A antibody [6F10] images

  • ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
  • All lanes : Anti-Surfactant Protein A antibody [6F10] (ab51891) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
  • ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.

References for Anti-Surfactant Protein A antibody [6F10] (ab51891)

This product has been referenced in:
  • He X  et al. Identification of a nanobody specific to human pulmonary surfactant protein A. Sci Rep 7:1412 (2017). IHC-Fr, ICC/IF ; Human . Read more (PubMed: 28469136) »
  • Nita I  et al. Hepatocyte growth factor secreted by bone marrow stem cell reduce ER stress and improves repair in alveolar epithelial II cells. Sci Rep 7:41901 (2017). IF . Read more (PubMed: 28157203) »

See all 8 Publications for this product

Product Wall

Application
Western blot
Sample
Rabbit Tissue lysate - whole (lung)
Loading amount
25 µg
Specification
lung
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Mun-Suk Park

Verified customer

Submitted Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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