Anti-Surfactant Protein A antibody (ab78173)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab78173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
ELISA 1/1000 - 1/30000.

Target

  • Cellular localizationSecreted protein; extracellular space.
  • Database links
  • Alternative names
    • 35 kDa pulmonary surfactant-associated protein antibody
    • Alveolar proteinosis protein antibody
    • COLEC4 antibody
    • Collectin 4 antibody
    • PSAP antibody
    • PSPA antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant-associated protein A1 antibody
    • SFTP1 antibody
    • SFTPA antibody
    • SFTPA1 antibody
    • SFTPA1B antibody
    • SP A antibody
    • SP A1 antibody
    see all

Anti-Surfactant Protein A antibody images

  • All lanes : Anti-Surfactant Protein A antibody (ab78173) at 1/1000 dilution

    Lane 1 : Cow lung tissue lysate
    Lane 2 : Cow lung tissue lysate
    Lane 3 : Cow lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

References for Anti-Surfactant Protein A antibody (ab78173)

ab78173 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Tissue lysate - whole (lung)
Loading amount 20 µg
Specification lung
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Submitted Jan 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"