Anti-Surfactant protein D antibody [12G5] (ab17781)

Overview

  • Product nameAnti-Surfactant protein D antibody [12G5]
    See all Surfactant protein D primary antibodies
  • Description
    Mouse monoclonal [12G5] to Surfactant protein D
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Recombinant neck CRD surfactant protein D (Human).

  • Positive control
    • Epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder.

Properties

Applications

Our Abpromise guarantee covers the use of ab17781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500. For 16 hrs on perinatal mouse lung samples (see Abreview for further details).
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
WB 1/20000. Predicted molecular weight: 37 kDa. ab17781 reacts strongly with Surfactant protein D, both in reduced and non-reduced forms.
IHC-P 1/800.

Target

  • FunctionContributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similaritiesBelongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links
  • Alternative names
    • COLEC 7 antibody
    • COLEC7 antibody
    • Collectin-7 antibody
    • Collectin7 antibody
    • Lung surfactant protein D antibody
    • PSP D antibody
    • PSP-D antibody
    • PSP-D Surfactant protein D antibody
    • PSPD antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant associated protein D antibody
    • Pulmonary surfactant associated protein PSP-D antibody
    • Pulmonary surfactant-associated protein D antibody
    • SFTP 4 antibody
    • SFTP4 antibody
    • SFTPD antibody
    • SFTPD_HUMAN antibody
    • SP D antibody
    • SP-D antibody
    • Surfactant associated protein pulmonary 4 antibody
    • Surfactant pulmonary associated protein D antibody
    see all

Anti-Surfactant protein D antibody [12G5] images

  • ab17781 staining Surfactant protein D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • ab17781 staining Surfactant protein D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab17781 at a 1/500 dilution staining perinatal mouse lung tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2.5% BSA for 30 minutes. The antibody was incubated with the tissue for 16 hours and bound antibody was detected with an Alexa-Fluor ® 594 conjugated Goat anti-Mouse IgG antibody.

    This image is courtesy of an Anonymous Abreview submitted on 3 April 2006.

    See Abreview

References for Anti-Surfactant protein D antibody [12G5] (ab17781)

ab17781 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Roti Immunoblock as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 24°C
Sample Human Cell (differentiated amniotic epithelial cell)
Specification differentiated amniotic epithelial cell
Permeabilization Yes - 0.25 % Triton X-100
Fixative Formaldehyde
Username

Ms. Angela Lemke

Verified customer

Submitted Mar 31 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human gall bladder)
Specification human gall bladder
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Mrs. Maria Cecilia Ybanez

Verified customer

Submitted Mar 24 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human gall bladder)
Specification human gall bladder
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Mar 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Cow Tissue sections (pancreatic ductal cells)
Specification pancreatic ductal cells
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Nov 04 2013

Thank you for your phone call earlier today. I checked about the Surfactant protein D antibody you were looking for. We currently have several mouse monoclonal antibodies that may be suitable: ab17781, ab15696, ab15695 and ab15687 for example. I hope t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (perinatal mouse lung)
Specification perinatal mouse lung
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2.5%
Username

Abcam user community

Verified customer

Submitted Apr 03 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"