Anti-Surfactant protein D antibody [VI F11] (ab15688)


  • Product nameAnti-Surfactant protein D antibody [VI F11]
    See all Surfactant protein D primary antibodies
  • Description
    Mouse monoclonal [VI F11] to Surfactant protein D
  • SpecificityAlveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Purified rat surfactant protein D from bronchoalveolar lavage.

  • EpitopeThe VIF11 epitope is localized in the lectin domain of Surfactant protein D.
  • Positive control
    • Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-.



Our Abpromise guarantee covers the use of ab15688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at a concentration of 2 µg/ml.
    IHC-P: 1/20 (human) - 1/400 (rat).
    WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionContributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
    • Sequence similaritiesBelongs to the SFTPD family.
      Contains 1 C-type lectin domain.
      Contains 1 collagen-like domain.
    • Post-translational
      The N-terminus is blocked.
      Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
    • Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
    • Information by UniProt
    • Database links
    • Alternative names
      • COLEC 7 antibody
      • COLEC7 antibody
      • Collectin-7 antibody
      • Collectin7 antibody
      • Lung surfactant protein D antibody
      • PSP D antibody
      • PSP-D antibody
      • PSP-D Surfactant protein D antibody
      • PSPD antibody
      • Pulmonary surfactant apoprotein antibody
      • Pulmonary surfactant associated protein D antibody
      • Pulmonary surfactant associated protein PSP-D antibody
      • Pulmonary surfactant-associated protein D antibody
      • SFTP 4 antibody
      • SFTP4 antibody
      • SFTPD antibody
      • SFTPD_HUMAN antibody
      • SP D antibody
      • SP-D antibody
      • Surfactant associated protein pulmonary 4 antibody
      • Surfactant pulmonary associated protein D antibody
      see all

    References for Anti-Surfactant protein D antibody [VI F11] (ab15688)

    This product has been referenced in:
    • Yadav AK  et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One 9:e108815 (2014). WB ; Human . Read more (PubMed: 25303045) »
    • Kasper M  et al. Monoclonal antibodies to surfactant protein D: evaluation of immunoreactivity in normal rat lung and in a radiation-induced fibrosis model. Exp Lung Res 21:577-88 (1995). Read more (PubMed: 7588444) »

    See all 2 Publications for this product

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    Thank you for this additional information. A few suggestions just to follow a protocol a little more similar to how we have tested. We generally recommend blocking in 5% milk in TBST and incubating the primary antibody overnight at 4 degrees. You may a...

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