Anti-Surfactant protein D antibody [VIF11] (Biotin) (ab15696)

Overview

  • Product nameAnti-Surfactant protein D antibody [VIF11] (Biotin)
    See all Surfactant protein D primary antibodies
  • Description
    Mouse monoclonal [VIF11] to Surfactant protein D (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified from bronchoalveolar lavage) (Rat).

  • EpitopeLocalised in the lectin domain of Surfactant protein D.
  • Positive control
    • Alveolar type II cells,alveolar macrophages. Clara cells SP-A+ and SP-A-.

Properties

Applications

Our Abpromise guarantee covers the use of ab15696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10 - 1/200. 1/10 for staining human tissue, 1/200 for staining rat tissue.
IHC-P 1/10 - 1/200. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. 1/10 for staining human tissue, 1/200 for staining rat and mouse tissue (see Abreview).
ELISA Use a concentration of 2 µg/ml.

Target

  • FunctionContributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similaritiesBelongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links
  • Alternative names
    • COLEC 7 antibody
    • COLEC7 antibody
    • Collectin-7 antibody
    • Collectin7 antibody
    • Lung surfactant protein D antibody
    • PSP D antibody
    • PSP-D antibody
    • PSP-D Surfactant protein D antibody
    • PSPD antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant associated protein D antibody
    • Pulmonary surfactant associated protein PSP-D antibody
    • Pulmonary surfactant-associated protein D antibody
    • SFTP 4 antibody
    • SFTP4 antibody
    • SFTPD antibody
    • SFTPD_HUMAN antibody
    • SP D antibody
    • SP-D antibody
    • Surfactant associated protein pulmonary 4 antibody
    • Surfactant pulmonary associated protein D antibody
    see all

Anti-Surfactant protein D antibody [VIF11] (Biotin) images

  • ab15696 at a 1/200 dilution staining Surfactant protein D from mouse lung tissue sections by Immunohistochemistry (frozen sections). The antibody was incubated with the tissue for 24 hours and then detected using an Alexa Fluor® 488 conjugated Goat anti-mouse IgG antibody.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 24 January 2006.

    See Abreview

References for Anti-Surfactant protein D antibody [VIF11] (Biotin) (ab15696)

This product has been referenced in:
  • Schneider C  et al. Induction of the nuclear receptor PPAR-? by the cytokine GM-CSF is critical for the differentiation of fetal monocytes into alveolar macrophages. Nat Immunol 15:1026-37 (2014). Read more (PubMed: 25263125) »
  • Kasper M  et al. Monoclonal antibodies to surfactant protein D: evaluation of immunoreactivity in normal rat lung and in a radiation-induced fibrosis model. Exp Lung Res 21:577-88 (1995). Read more (PubMed: 7588444) »

See all 2 Publications for this product

Product Wall

Thank you for your phone call earlier today. I checked about the Surfactant protein D antibody you were looking for. We currently have several mouse monoclonal antibodies that may be suitable: ab17781, ab15696, ab15695 and ab15687 for example. I hope t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5
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Submitted Jan 24 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"