Anti-Survivin antibody [60.11] (ab93274)


  • Product nameAnti-Survivin antibody [60.11]
    See all Survivin primary antibodies
  • Description
    Mouse monoclonal [60.11] to Survivin
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human Survivin.



Our Abpromise guarantee covers the use of ab93274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.


  • FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similaritiesBelongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
  • DomainThe BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

Anti-Survivin antibody [60.11] images

  • Overlay histogram showing HeLa cells stained with ab93274 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93274, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Survivin antibody [60.11] (ab93274)

ab93274 has not yet been referenced specifically in any publications.

Product Wall

Yes, our standard western blotting conditions are reduced and denatured, so we would recommend running under those conditions.

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Unfortunately, this antibody has not been epitope-mapped, so we do not know exactly where it is binding.

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I apologize for delay as I was gathering temperature sensitivity data for this antibody. Unfortunately laboratory is not aware of any kind of degradation of this antibody after storing it at -20C. We however acknowled...

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As per your previous email you were quite happy with the antibody as it was working. Could you let me know what is the problem now? Do you mean the aliquots does not work after storing the antibody at -20C? Do you know if all the aliquots failed or it ...

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We unfortunately do not have new batch available yet. Would like to try another vial from same batch?

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The ab7170 antibody is out of stock which is why it was unpublished. I am sorry we will not be able to provide this product. The product ...

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