Recombinant full length protein corresponding to Human Survivin .
Database link: O15392
Our Abpromise guarantee covers the use of ab469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250. See Abreview by William Moore; fix with formaldehyde.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.|
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration. Recommended to use at 5-7µg/ml.|
|RIP||Use at an assay dependent concentration. PubMed: 19542185|
Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 µg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
HeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK.
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
ab469 immunoprecipitating Survivin from HeLa cell lysate. HeLa cells were lysed after colcemid block (ON) and immunoprecipitated with ab469 at a 1/1000 dilution. HeLa cells clear lysate (Lane 1) as well as the bound material (Lane 2) were loaded on a 15 % acrylamide gel. An HRP conjugated Donkey Anti-rabbit IgG was used as the secondary antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 12 April 2006.