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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-Survivin antibody
See all Survivin products (18) ...
Description
Rabbit polyclonal to Survivin
Specificity
Specific for survivin.
Tested applications
ICC/IF, WB, IHC-P, IP, RIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Cat, Dog, Human
Immunogen
Recombinant full length protein (Human).
Positive control
WB: HeLa cell lysate ICC: HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/250(See Abreview by William Moore; fix with formaldehyde.)
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 16 kDa.(Found to work at 1/5000 dilution.)
IHC-P: Use a concentration of 0.5 µg/mlPerform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution. (Recommended to use at 5-7µg/ml.)
RIP: Use at an assay dependent dilution. (PubMed: 19542185)
Target
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis.
Domain
The BIR repeat is necessary and sufficient for HBXIP binding.
Post-translational
modifications
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionO15392
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- API4 antibody
- Apoptosis Inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Database links
- Entrez Gene: 493835 Cat
- Entrez Gene: 414925 Cow
- Entrez Gene: 442936 Dog
- Entrez Gene: 332 Human
- Entrez Gene: 11799 Mouse
- Entrez Gene: 64041 Rat
- Omim: 603352 Human
- SwissProt: Q6I6F4 Cat
see all
Anti-Survivin antibody images:
Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab469)
HeLa cells in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK.
William Moore, Org unknown, Country unknown
Western blot - Survivin antibody (ab469)
All lanes : Anti-Survivin antibody (ab469) at 1 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab469)
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
Immunoprecipitation - Survivin antibody (ab469)
ab469 immunoprecipitating Survivin from HeLa cell lysate. HeLa cells were lysed after colcemid block (ON) and immunoprecipitated with ab469 at a 1/1000 dilution. HeLa cells clear lysate (Lane 1) as well as the bound material (Lane 2) were loaded on a 15 % acrylamide gel. An HRP conjugated Donkey Anti-rabbit IgG was used as the secondary antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 12 April 2006.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Survivin antibody (ab469)
ab469 staining Survivin from Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Ben Davidson
Protocols
References for Anti-Survivin antibody (ab469)
This product has been referenced in:
- Kaminskyy VOet al. Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL. Carcinogenesis 32:1450-8 (2011).Read more (PubMed: 21771726) »
- Dallaglio Ket al. Endogenous survivin modulates survival and proliferation in UVB-treated human keratinocytes. Exp Dermatol 18:464-71 (2009). WB; Human.Read more (PubMed: 19320741) »
See all 35 publications for this product
Publishing research using ab469? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"