Overview

  • Product nameAnti-Survivin antibody [EP2880Y]
    See all Survivin primary antibodies
  • Description
    Rabbit monoclonal [EP2880Y] to Survivin
  • Tested applicationsSuitable for: IHC-P, WB, IP, Flow Cyt, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Survivin aa 1-100 (N terminal).

  • Positive control
    • HeLa and Jurkat lysates; human urinary bladder carcinoma and human colonic adenocarcinoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Survivin antibody (Alexa Fluor® 488) [EP2880Y] (ab204264)
    Anti-Survivin antibody (Alexa Fluor® 647) [EP2880Y] (ab204464)

    Anti-Survivin antibody (Alexa Fluor® 594) [EP2880Y] (ab207879)

Properties

Applications

Our Abpromise guarantee covers the use of ab76424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Signal amplification system might be necessary or the use of polymerized HRP secondary antibodies.
WB 1/5000 - 1/20000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/10.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Survivin (ab27468). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab27468 as Capture.

Target

  • FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similaritiesBelongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
  • DomainThe BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

Anti-Survivin antibody [EP2880Y] images

  • Immunofluorescent staining of MCF-7 cells labelling Bcl-2 with purified ab76424 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue). 

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • All lanes : Anti-Survivin antibody [EP2880Y] (ab76424) at 1/10000 dilution

    Lane 1 : HeLa lysates
    Lane 2 : Jurkat lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • ab76424 at 1/250 dilution staining Survivin in human urinary bladder carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
  • ab76424 at 1/250 dilution staining Survivin in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
  • ab76424 showing positive staining in Breast carcinoma tissue.

  • ab76424 showing positive staining in Cervical carcinoma tissue.

  • ab76424 showing positive staining in Fetal kidney tissue.

  • ab76424 showing positive staining in Fetal liver tissue.

  • ab76424 showing positive staining in Normal tonsil tissue.

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling Survivin with ab76424 at 1/100. Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature. Cells were incubated with the primary antibody overnight. An Alexa Fluor® 555-conjugated anti-rabbit IgG was used as the secondary antibody. Left - Survivin, Right - DAPI.

  • Overlay histogram showing HeLa cells stained with ab76424 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76424, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

  • Standard curve for Survivin (Analyte: ab87202); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab27468 at 1µg/ml and Detector Antibody abRabbit monoclonal [EP2880Y] to Survivin (ab76424) at 0.5µg/ml. Concentration of Rabbit monoclonal [EP2880Y] to Survivin (ab76424) may vary from lot to lot; please use this curve as guideline.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Protocols

References for Anti-Survivin antibody [EP2880Y] (ab76424)

This product has been referenced in:
  • Lin Y  et al. Survivin is expressed in degenerated nucleus pulposus cells and is involved in proliferation and the prevention of apoptosis in vitro. Mol Med Rep 13:1026-32 (2016). WB ; Human . Read more (PubMed: 26648308) »
  • Peng HY  et al. MPT0B098, a Microtubule Inhibitor, Suppresses JAK2/STAT3 Signaling Pathway through Modulation of SOCS3 Stability in Oral Squamous Cell Carcinoma. PLoS One 11:e0158440 (2016). WB ; Human . Read more (PubMed: 27367272) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Sample Human Tissue sections (human glioblastoma xenograft in nude mice)
Specification human glioblastoma xenograft in nude mice
Permeabilization Yes - Triton 0.025%
Fixative Alcool-Formol-Acetate (AFA)
Username

Abcam user community

Verified customer

Submitted Jul 26 2013

Thank you very much for your call earlier this week, and I apologize for the delay in getting this information for you.

The antibody ab76424 was used at a 1:2000 dilution in sandwich ELISA.

I hope that this will be useful, but pleas...

Read More

Thank you very much for your email.

xxx is currently not in the office but I will pass the message on. I'm sure she will be very pleased.

If we can be of any further assistance, please do not hesitate to let me know.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital...

Read More

Thank you for contacting Abcam regarding ab76424.


This antibody is predicted to cross-react with all 7 isoforms of survivin, based on 100% sequence homology.


I hope this information is helpful. Please let me know if you ...

Read More

Thank you for your inquiry.

I can confirm that the immunogen maps to the first 25 amino acids of human Survivin, on the N-terminus (UniProt: O15392).

Therefore the antibody should recognise all isoforms since they do not differ in t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma Initiating Cells (Primary cells))
Loading amount 30 µg
Specification Glioblastoma Initiating Cells (Primary cells)
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Thank you for contacting Abcam regarding ab76424 and ab3731. I am sorry that you have been experiencing difficulties getting these antibodies to work in WB.  I am following up with the lab regarding reactivity of ab76424 with Delta Ex3.  Regarding a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Ovarian cancer cells OVCAR-3)
Loading amount 40 µg
Specification Ovarian cancer cells OVCAR-3
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Epithelial cell)
Loading amount 30 µg
Specification Epithelial cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"