Anti-Survivin antibody [SP79] (ab93725)

Overview

• Product name
  Anti-Survivin antibody [SP79]
  See all Survivin primary antibodies
• Description
  Rabbit monoclonal [SP79] to Survivin
• Tested applications
  Suitable for: IHC-Pmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to C terminal residues of Human Survivin.

• Positive control
  • Human stomach tissue.
• General notes

  This product is a recombinant rabbit monoclonal antibody.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C. Do Not Freeze.
• Storage buffer
  Preservative: Sodium Azide
  Constituents: BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Monoclonal
• Clone number
  SP79
• Isotype
  IgG
• Research areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Survivin / IAPs

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Astrocyte marker

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Death receptors & ligands
  • IAPs

  • Cancer
  • Cell Death
  • Apoptosis
  • Receptors
  • Death receptors & ligands
  • IAPs

Applications

Target

• Function
  Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
• Tissue specificity
  Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
• Sequence similarities
  Belongs to the IAP family.
  Contains 1 BIR repeat.
• Developmental stage
  Expression is cell cycle-dependent and peaks at mitosis.
• Domain
  The BIR repeat is necessary and sufficient for HBXIP binding.
• Post-translational
  modifications
  Ubiquitination is required for centrosomal targeting.
  In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
• Cellular localization
  Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.

• Target information above from: UniProt accession O15392 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 332 Human
  • Omim: 603352 Human
  • SwissProt: O15392 Human
  • Unigene: 514527 Human
• Alternative names
  • API4 antibody
  • Apoptosis inhibitor 4 antibody
  • Apoptosis inhibitor survivin antibody

  • Apoptosis inhibitor4 antibody
  • Baculoviral IAP repeat containing 5 antibody
  • Baculoviral IAP repeat containing protein 5 antibody
  • Baculoviral IAP repeat-containing protein 5 antibody
  • BIRC 5 antibody
  • BIRC5 antibody
  • BIRC5_HUMAN antibody
  • EPR 1 antibody
  • IAP4 antibody
  • Survivin variant 3 alpha antibody
  • SVV antibody
  • TIAP antibody

  see all

Protocols

• Immunohistochemistry protocols