Anti-Survivin (phospho T117) antibody (ab63514)

Overview

  • Product nameAnti-Survivin (phospho T117) antibody
    See all Survivin primary antibodies
  • Description
    Rabbit polyclonal to Survivin (phospho T117)
  • SpecificityThis antibody detects endogenous levels of Survivin only when phosphorylated at threonine 117.
  • Tested applicationsSuitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized phosphopeptide derived from human Survivin around the phosphorylation site of threonine 117

  • Positive control
    • A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similaritiesBelongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
  • DomainThe BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

Anti-Survivin (phospho T117) antibody images

  • ab63514 at a 1:500 dilution staining Survivin in A549 cells.
    Left image un-treated.
    Right image treated with phosphopeptide.

References for Anti-Survivin (phospho T117) antibody (ab63514)

ab63514 has not yet been referenced specifically in any publications.

Product Wall

Thank you for the update. The phospho-survivin antibody is likely cross-reacting with another phosphoprotein. I am not sure what to suggest to get rid of the background, and in any case, I think even the staining of what appear to be vessels is a littl...

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Thank you for the photo. Can you please tell me what the tissue is, and the species?

For your immunostain, did you include a negative control of just secondary antibody, no survivin antibody, to confirm that the survivin antibody is the sour...

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I am sorry to read that these three antibodies are giving you trouble.

We do not have western data for the survivin antibody and so do not have a tested protocol but hopefully I can make some suggestions. Under the terms of our guarantee, th...

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