The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceKMT1 / Clr4 methylates K9 of histone H3. H3 K9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones. Targeting to histone probably involves Clr3 and Rik1. Essential for silencing of centromeres and directional switching of the mating type.