Anti-SUZ12 antibody - ChIP Grade, purified (ab195499)

Overview

  • Product name
    Anti-SUZ12 antibody - ChIP Grade, purified
    See all SUZ12 primary antibodies
  • Description
    Rabbit polyclonal to SUZ12 - ChIP Grade, purified
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human SUZ12 (N terminal).
    Database link: Q15022

  • Positive control
    • ChIP: Mouse ES cells. WB: HeLa nuclear extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab195499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.

10 μg/ChIP

WB 1/1000. Predicted molecular weight: 83 kDa.

Target

  • Function
    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Tissue specificity
    Overexpressed in breast and colon cancer.
  • Involvement in disease
    Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
  • Sequence similarities
    Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
    Contains 1 C2H2-type zinc finger.
  • Developmental stage
    Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ChET 9 protein antibody
    • CHET9 antibody
    • Chromatin precipitated E2F target 9 protein antibody
    • JJAZ1 antibody
    • Joined to JAZF1 protein antibody
    • KIAA0160 antibody
    • Polycomb protein SUZ12 antibody
    • Suppressor of zeste 12 homolog antibody
    • Suppressor of zeste 12 protein homolog antibody
    • SUZ12 antibody
    • SUZ12 polycomb repressive complex 2 subunit antibody
    • SUZ12_HUMAN antibody
    see all

Images

  • ChIP analysis using Mouse ES cells, labeling SUZ12 with ab195499 at 10 µg. ChIP was performed using 600 µg chromatin. IgG (10 μg) was used as negative IP control.  The IP’d DNA was analysed by QPCR with specific primer sets for different positive and negative genes. The results are expressed as a % of input (the relative abundance of target DNA in the immunoprecipitates compared to the input DNA).

  • Anti-SUZ12 antibody - ChIP Grade, purified (ab195499) at 1/1000 dilution (in TBS-Tween containing 5% skimmed milk) + HeLa nuclear extracts at 20 µg

    Predicted band size: 83 kDa

References

ab195499 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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