Overview

  • Product nameAnti-SV2A antibody
    See all SV2A primary antibodies
  • Description
    Goat polyclonal to SV2A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Horse, Cow, Dog, Human, Pig, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-TVELYPSDKRTTA

    , corresponding to internal sequence amino acids 673-685 of Human SV2A (NP_055664.2)

  • Positive control
    • Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 83 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
    • Sequence similaritiesBelongs to the major facilitator superfamily.
    • Post-translational
      modifications
      Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
      N-glycosylated.
    • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0736 antibody
      • OTTHUMP00000014065 antibody
      • SV2 antibody
      • Sv2a antibody
      • SV2A_HUMAN antibody
      • Synaptic vesicle glycoprotein 2 antibody
      • Synaptic vesicle glycoprotein 2A antibody
      • Synaptic vesicle protein 2a antibody
      see all

    Anti-SV2A antibody images

    • Anti-SV2A antibody (ab77177) at 0.3 µg/ml + rat brain lysate in RIPA buffer at 35 µg

      Predicted band size : 83 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-SV2A antibody (ab77177)

    ab77177 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77177.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"