Overview

  • Product name
  • Description
    Goat polyclonal to SV2A
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Horse, Cow, Dog, Human, Pig, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-TVELYPSDKRTTA

    , corresponding to internal sequence amino acids 673-685 of Human SV2A (NP_055664.2)

  • Positive control
    • Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 83 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
    • Sequence similarities
      Belongs to the major facilitator superfamily.
    • Post-translational
      modifications
      Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
      N-glycosylated.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0736 antibody
      • OTTHUMP00000014065 antibody
      • SV2 antibody
      • Sv2a antibody
      • SV2A_HUMAN antibody
      • Synaptic vesicle glycoprotein 2 antibody
      • Synaptic vesicle glycoprotein 2A antibody
      • Synaptic vesicle protein 2a antibody
      see all

    Images

    • Anti-SV2A antibody (ab77177) at 0.3 µg/ml + rat brain lysate in RIPA buffer at 35 µg

      Predicted band size : 83 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    References

    ab77177 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77177.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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