Overview

  • Product name
    Anti-SV2B antibody
  • Description
    Mouse polyclonal to SV2B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SV2B (NP_055663.1, 1 a.a. ~ 683 a.a) full-length human protein.

  • Positive control
    • transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).

Target

  • Relevance
    SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. While SV2A is expressed ubiquitously throughout the brain, SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern.
  • Cellular localization
    Cytoplasmic vesicle, secretory vesicle. Synaptic vesicle membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • Synaptic vesicle protein 2B antibody

Images

  • All lanes : Anti-SV2B antibody (ab68025) at 1/500 dilution

    Lane 1 : SV2B transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

ab68025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab68025.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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