Anti-SV40 T-antigen antibody [PAb416] (ab16879)
Key features and details
- Mouse monoclonal [PAb416] to SV40 T-antigen
- Suitable for: ICC/IF, WB
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-SV40 T-antigen antibody [PAb416]
See all SV40 T-antigen primary antibodies -
Description
Mouse monoclonal [PAb416] to SV40 T-antigen -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
SV40 transformed mouse cells
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Epitope
Within amino acids 83-128 of the large T-antigen. -
Positive control
- SV80 or SV-T2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PAb416 -
Myeloma
NS1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use a concentration of 1 - 5 µg/ml.
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WB | (4) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
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Notes |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. |
Target
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Cellular localization
Nuclear -
Alternative names
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
Images
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All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
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ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab16879 has been referenced in 35 publications.
- Kazemian A et al. Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells. Animals (Basel) 12:N/A (2022). PubMed: 35405788
- McIlroy D et al. Persistent BK Polyomavirus Viruria is Associated with Accumulation of VP1 Mutations and Neutralization Escape. Viruses 12:N/A (2020). PubMed: 32751274
- Low JY et al. Stromal CAVIN1 Controls Prostate Cancer Microenvironment and Metastasis by Modulating Lipid Distribution and Inflammatory Signaling. Mol Cancer Res 18:1414-1426 (2020). PubMed: 32493699
- Ferreira CB et al. Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity. Mol Ther Methods Clin Dev 17:209-219 (2020). PubMed: 31970199
- Graubner FR et al. Canine decidualization in vitro: extracellular matrix modification, progesterone mediated effects and selective blocking of prostaglandin E2 receptors. J Reprod Dev 66:319-329 (2020). PubMed: 32201411