Overview

  • Product nameAnti-SV40 VP1 antibody
  • Description
    Rabbit polyclonal to SV40 VP1
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Simian Virus 40 (SV40)
  • Immunogen

    Recombinant fusion protein

Properties

Applications

Our Abpromise guarantee covers the use of ab53977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.

Target

  • RelevanceVP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.
  • Alternative names
    • Capsid protein VP1 antibody

Anti-SV40 VP1 antibody images

  • All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution

    Lane 1 : BK virus infected 293TT whole cell lysate
    Lane 2 : Mock infected 293TT whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    See Abreview



  • Predicted band size : 40 kDa
    SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
  • ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.
  • ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.

References for Anti-SV40 VP1 antibody (ab53977)

This product has been referenced in:
  • Henriksen S  et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus. J Virol 88:7556-68 (2014). Human . Read more (PubMed: 24760884) »
  • Stergiou L  et al. Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. PLoS One 8:e55799 (2013). WB ; Human . Read more (PubMed: 23409046) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (BK virus infected 293TT cells)
Specification BK virus infected 293TT cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Nov 18 2014

Thank you for your enquiry and for your patience. We aim to get back to customers within 24 hours and I am sorry this did not happen on this occasion.

I have attached the MSDS for ab53977. This is now also available on the datasheet.
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Hello!

The supplier of the antibody was unable to provide a concentration, although I can tell you for rabbit antibodies typically the IgG is 12 - 15 mg/ml. If you did need a more specific concentration you could purify the antibody with a ki...

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Thank you for your enquiry. I can confirm that ab53977 SV40 VP1 antibody detects infecting SV40 in the cell during the first hours of infection, before new VP1 is expressed from the incoming virus. I am sorry it has not yet been tested tested i...

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Thank you for your enquiry. I have received confirmation from the originator of the product that this ab53977 do cross-react with JC and BK, and in-fact give very good signals with pathological specimens. However, it cannot be used to distinguish b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"