Overview

  • Product name
  • Description
    Goat polyclonal to SWAP70
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: EEREKNWKEKKTTE, corresponding to C terminal amino acids 572-585 of Human SWAP70.

  • Positive control
    • Daudi lysate.
  • General notes
    Principal Names – SWAP70; SWAP-70 protein; SWAP-70; KIAA0640; KIAA0640 protein. Official Gene Symbol - SWAP70 (interim symbol). GenBank Accession Number – XP_049197. LocusLink ID - 23075

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Western Blot – Approx 70kDa band seen in Daudi lysate. Recommended for use at 1.0-3.0ug/ml.
  • Target

    • Function
      Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
    • Tissue specificity
      Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
    • Sequence similarities
      Contains 1 PH domain.
    • Domain
      The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • Post-translational
      modifications
      Tyrosine-phosphorylated.
    • Cellular localization
      Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0640 antibody
      • 70kDa antibody
      • AV235546 antibody
      • FLJ39540 antibody
      • HSPC321 antibody
      • SWA 70 protein antibody
      • SWAP 70 antibody
      • SWAP switching B-cell complex 70kDa subunit antibody
      • SWAP-70 antibody
      • SWAP70 antibody
      • Switch associated protein 70 antibody
      • Switch-associated protein 70 antibody
      • SWP70_HUMAN antibody
      see all

    Images

    • ab2511 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

    References

    ab2511 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab2511.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up