Overview

  • Product nameAnti-SWAP70 antibody
    See all SWAP70 primary antibodies
  • Description
    Goat polyclonal to SWAP70
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: EEREKNWKEKKTTE, corresponding to C terminal amino acids 572-585 of Human SWAP70.

  • Positive control
    • Daudi lysate.
  • General notesPrincipal Names – SWAP70; SWAP-70 protein; SWAP-70; KIAA0640; KIAA0640 protein. Official Gene Symbol - SWAP70 (interim symbol). GenBank Accession Number – XP_049197. LocusLink ID - 23075

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot – Approx 70kDa band seen in Daudi lysate. Recommended for use at 1.0-3.0ug/ml.
  • Target

    • FunctionPhosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
    • Tissue specificityExpressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
    • Sequence similaritiesContains 1 PH domain.
    • DomainThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • Post-translational
      modifications
      Tyrosine-phosphorylated.
    • Cellular localizationCytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0640 antibody
      • 70kDa antibody
      • AV235546 antibody
      • FLJ39540 antibody
      • HSPC321 antibody
      • SWA 70 protein antibody
      • SWAP 70 antibody
      • SWAP switching B-cell complex 70kDa subunit antibody
      • SWAP-70 antibody
      • SWAP70 antibody
      • Switch associated protein 70 antibody
      • Switch-associated protein 70 antibody
      • SWP70_HUMAN antibody
      see all

    Anti-SWAP70 antibody images

    • ab2511 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

    References for Anti-SWAP70 antibody (ab2511)

    ab2511 has not yet been referenced specifically in any publications.

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