Overview

  • Product nameAnti-SWAP70 antibody
    See all SWAP70 primary antibodies
  • Description
    Rabbit polyclonal to SWAP70
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide, sequence taken from within amino acids 300-350 of human SWAP-70.

  • Positive control
    • Daudi cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 69 kDa).

Target

  • FunctionPhosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
  • Tissue specificityExpressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
  • Sequence similaritiesContains 1 PH domain.
  • DomainThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
  • Post-translational
    modifications
    Tyrosine-phosphorylated.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0640 antibody
    • 70kDa antibody
    • AV235546 antibody
    • FLJ39540 antibody
    • HSPC321 antibody
    • SWA 70 protein antibody
    • SWAP 70 antibody
    • SWAP switching B-cell complex 70kDa subunit antibody
    • SWAP-70 antibody
    • SWAP70 antibody
    • Switch associated protein 70 antibody
    • Switch-associated protein 70 antibody
    • SWP70_HUMAN antibody
    see all

Anti-SWAP70 antibody images

  • Anti-SWAP70 antibody (ab47084) at 2 µg/ml + Daudi cell lysate

    Predicted band size : 69 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SWAP70 antibody (ab47084)

ab47084 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"