Anti-Swi6 antibody (ab188276)
Key features and details
- Rabbit polyclonal to Swi6
- Suitable for: WB, IP, ChIP
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
Overview
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Product name
Anti-Swi6 antibody -
Description
Rabbit polyclonal to Swi6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Schizosaccharomyces pombe -
Immunogen
Recombinant full length protein corresponding to Schizosaccharomyces pombe Swi6 aa 1 to the C-terminus.
Database link: P40381 -
Positive control
- S. pombe Swi6 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
Target
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Relevance
The chromatin structure of Fission yeast centromeres is regulated epigenetically. In the central core domain, the histone H3 variant Cnp1(CENP-A) plays a key role. In the flanking heterochromatin domain, histones are kept underacetylated by the histone deacetylases (HDACs) Clr3, Clr6 and Sir2, and methylated by Clr4 methyltransferase (HMTase) to create a specific binding site for the Swi6 protein. Swi6 then directly mediates cohesin binding to the centromeric heterochromatin. Recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Involved in the repression of the silent mating-type loci MAT2 and MAT3. May compact MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 interacts with histone H3 methylated at Lys-9 and with the cohesin subunit psc3. Swi6 associates with Swi4 to activate HO and many other late G1-specific transcripts in budding yeast. In contrast, histone H3 methylated at Lys9 and its interacting protein Swi6/HP1, which define heterochromatin, coated extended domains associated with a variety of repeat elements and small islands corresponding to meiotic genes. Contains two chromo domains. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2541633 Schizosaccharomyces pombe
- SwissProt: P40381 Schizosaccharomyces pombe
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Alternative names
- Chromatin associated protein swi6 antibody
- Chromodomain protein Swi6 antibody
- SPAC664.01c antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab188276 has been referenced in 4 publications.
- Lim KK et al. Fission Yeast Methylenetetrahydrofolate Reductase Ensures Mitotic and Meiotic Chromosome Segregation Fidelity. Int J Mol Sci 22:N/A (2021). PubMed: 33440639
- Lei B et al. A Synthetic Approach to Reconstruct the Evolutionary and Functional Innovations of the Plant Histone Variant H2A.W. Curr Biol 31:182-191.e5 (2021). PubMed: 33096036
- Murawska M et al. The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states. Cell Rep 37:109944 (2021). PubMed: 34731638
- Sadeghi L et al. The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast. EMBO Rep 16:1673-87 (2015). ChIP ; Schizosaccharomyces pombe . PubMed: 26518661