• Product nameAnti-SYBL1 antibody [158.2]
    See all SYBL1 primary antibodies
  • Description
    Mouse monoclonal [158.2] to SYBL1
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Recombinant full SYBL1 protein (Human)

  • Positive control
    • extract from rat brain



Our Abpromise guarantee covers the use of ab36195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 19138172
WB 1/500 - 1/2000. Predicted molecular weight: 24 kDa.
IHC-Fr 1/10 - 1/400.
IHC-P Use at an assay dependent concentration.


  • FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
  • Tissue specificityDetected in all tissues tested.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ53045 antibody
    • FLJ53762 antibody
    • FLJ54296 antibody
    • HGNC:11486 antibody
    • OTTHUMP00000024258 antibody
    • OTTHUMP00000024259 antibody
    • OTTHUMP00000225953 antibody
    • SYBL 1 antibody
    • SYBL1 antibody
    • Synaptobrevin like 1 antibody
    • Synaptobrevin-like protein 1 antibody
    • Tetanus insensitive VAMP antibody
    • Tetanus neurotoxin insensitive VAMP antibody
    • Tetanus-insensitive VAMP antibody
    • TI VAMP antibody
    • Ti-VAMP antibody
    • TIVAMP antibody
    • VAMP-7 antibody
    • VAMP7 antibody
    • VAMP7_HUMAN antibody
    • Vesicle-associated membrane protein 7 antibody
    see all

Anti-SYBL1 antibody [158.2] images

  • Anti-SYBL1 antibody [158.2] (ab36195) at 1/500 dilution + Tissue lysates prepared from rat brain

    Predicted band size : 24 kDa
  • Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

References for Anti-SYBL1 antibody [158.2] (ab36195)

This product has been referenced in:
  • Pirooz SD  et al. UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs. Proc Natl Acad Sci U S A 111:2716-21 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24550300) »
  • Kukic I  et al. Zn2+ efflux through lysosomal exocytosis prevents Zn2+-induced toxicity. J Cell Sci 127:3094-103 (2014). Read more (PubMed: 24829149) »

See all 12 Publications for this product

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cell (Ovary (CHO-K1 - tissue culture))
Specification Ovary (CHO-K1 - tissue culture)
Fixative Formaldehyde
Permeabilization Yes - TX-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C

Mrs. Diana Thomas

Verified customer

Submitted Jun 03 2010