• Product name
    Anti-SYBL1 antibody [158.2]
    See all SYBL1 primary antibodies
  • Description
    Mouse monoclonal [158.2] to SYBL1
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Recombinant full SYBL1 protein (Human)

  • Positive control
    • extract from rat brain



Our Abpromise guarantee covers the use of ab36195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 19138172
WB 1/500 - 1/2000. Predicted molecular weight: 24 kDa.
IHC-Fr 1/10 - 1/400.
IHC-P Use at an assay dependent concentration.


  • Function
    Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
  • Tissue specificity
    Detected in all tissues tested.
  • Sequence similarities
    Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ53045 antibody
    • FLJ53762 antibody
    • FLJ54296 antibody
    • HGNC:11486 antibody
    • OTTHUMP00000024258 antibody
    • OTTHUMP00000024259 antibody
    • OTTHUMP00000225953 antibody
    • SYBL 1 antibody
    • SYBL1 antibody
    • Synaptobrevin like 1 antibody
    • Synaptobrevin-like protein 1 antibody
    • Tetanus insensitive VAMP antibody
    • Tetanus neurotoxin insensitive VAMP antibody
    • Tetanus-insensitive VAMP antibody
    • TI VAMP antibody
    • Ti-VAMP antibody
    • TIVAMP antibody
    • VAMP-7 antibody
    • VAMP7 antibody
    • VAMP7_HUMAN antibody
    • Vesicle-associated membrane protein 7 antibody
    see all


  • Anti-SYBL1 antibody [158.2] (ab36195) at 1/500 dilution + Tissue lysates prepared from rat brain

    Predicted band size : 24 kDa
  • Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.


This product has been referenced in:
  • Vogel GF  et al. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol 211:587-604 (2015). Read more (PubMed: 26553929) »
  • Pirooz SD  et al. UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs. Proc Natl Acad Sci U S A 111:2716-21 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24550300) »

See all 16 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. Your credit note ID is XXXX. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice o...

Read More
Immunocytochemistry/ Immunofluorescence
Chinese Hamster Cell (Ovary (CHO-K1 - tissue culture))
Ovary (CHO-K1 - tissue culture)
Yes - TX-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C

Mrs. Diana Thomas

Verified customer

Submitted Jun 03 2010


Sign up