Anti-SYCP2L antibody (ab122224)
Key features and details
- Rabbit polyclonal to SYCP2L
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SYCP2L antibody -
Description
Rabbit polyclonal to SYCP2L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: TSLICVIEDF FDTALIISRS SSEGKIQMLD SFLLSLGFLV TEKTVNHLLQ QEGLKTFNCI LHAVPREERK KFPLSEGMCH LMKDLARTLL TVGDYDQQV, corresponding to amino acids of Human SYCP2L.
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Positive control
- Human cerebral cortex tissue; A431 cells; RT 4, U 251 MG, Human liver and tonsil lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Tissue specificity
Expressed in the ovary (at protein level). -
Sequence similarities
Belongs to the SYCP2 family. -
Cellular localization
Nucleus. Detected in nuclear granules and sizable aggregates. - Information by UniProt
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Database links
- Entrez Gene: 221711 Human
- SwissProt: Q5T4T6 Human
- Unigene: 729128 Human
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Alternative names
- 145 kDa nucleolar protein homolog antibody
- C6orf177 antibody
- dJ62D2.1 antibody
see all
Images
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ab122224, at 1/200 dilution, staining SYCP2L in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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ab122224, at 4 µg/ml, staining SYCP2L in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
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All lanes : Anti-SYCP2L antibody (ab122224) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab122224 appears to be recognizing isoform 2 of Human SYCP2L (34kDa).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122224 has not yet been referenced specifically in any publications.