Overview

  • Product nameAnti-Syk antibody [EP573Y]
    See all Syk primary antibodies
  • Description
    Rabbit monoclonal [EP573Y] to Syk
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the protein kinase domain of human Syk.

  • Positive control
    • WB: Raji cell lysate. IHC-P: Paraffin embedded human lymph node.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-Syk antibody (Alexa Fluor® 488) [EP573Y] (ab198938)
    Anti-Syk antibody (Alexa Fluor® 647) [EP573Y] (ab198939)

    Anti-Syk antibody (HRP) [EP573Y] (ab198940)

Properties

Applications

Our Abpromise guarantee covers the use of ab40781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionNon-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen.
    • Tissue specificityWidely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.
    • DomainThe SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins. Some proteins like CLEC1B have a partial ITAM domain (also called hemITAM) containing a single YxxL motif. The interaction with SYK requires CLEC1B homodimerization.
    • Post-translational
      modifications
      Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
      Autophosphorylated. Phosphorylated on tyrosine residues by LYN following receptors engagement. Phosphorylation on Tyr-323 creates a binding site for CBL, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling. Phosphorylation at Tyr-348 creates a binding site for VAV1. Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway (By similarity). Phosphorylation on Ser-297 is very common, it peaks 5 minutes after BCR stimulation, and creates a binding site for YWHAG. Phosphorylation at Tyr-630 creates a binding site for BLNK. Dephosphorylated by PTPN6.
    • Cellular localizationCell membrane. Cytoplasm, cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.10.2 antibody
      • kinase Syk antibody
      • KSYK antibody
      • KSYK_HUMAN antibody
      • p72-Syk antibody
      • p72syk antibody
      • Spleen tyrosine kinase antibody
      • Syk antibody
      • Tyrosine protein kinase SYK antibody
      • Tyrosine-protein kinase SYK antibody
      see all

    Anti-Syk antibody [EP573Y] images



    • Predicted band size : 72 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: SYK knockout HAP1 cell lysate (40 µg)
      Lane 3: K562 cell lysate (40 µg)
      Lane 4: Raji cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab40781 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab40781 was shown to specifically react with Syk when Syk knockout samples were used. Wild-type and Syk knockout samples were subjected to SDS-PAGE. Ab40781 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-Syk antibody [EP573Y] (ab40781) at 1/20000 dilution + 10ug Raji cell lysate

      Predicted band size : 72 kDa
      Observed band size : 72 kDa

    References for Anti-Syk antibody [EP573Y] (ab40781)

    This product has been referenced in:
    • Ghotra VP  et al. SYK is a candidate kinase target for the treatment of advanced prostate cancer. Cancer Res 75:230-40 (2015). Read more (PubMed: 25388286) »
    • Blancato J  et al. SYK allelic loss and the role of Syk-regulated genes in breast cancer survival. PLoS One 9:e87610 (2014). ICC/IF ; Human . Read more (PubMed: 24523870) »

    See all 3 Publications for this product

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