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Recombinant fragment, corresponding to amino acids 5-360 of Human Syk.
Our Abpromise guarantee covers the use of ab3993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunofluorescence staining of Syk in human HeLa (human epithelial cell line from cervix adenocarcinoma) cell line using ab3993 (green) at 5 µg/ml. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunofluorescence staining of Syk in human primary fibroblasts using ab3993 (green) at 5 μg/ml. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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