Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)

Overview

  • Product nameAnti-Syk (phospho Y525) antibody [EP575(2)Y]
    See all Syk primary antibodies
  • Description
    Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525)
  • Specificityab62350 recognises Syk phosphorylated at Tyrosine 525
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surround Tyrosine 525 of Human Syk

  • Positive control
    • U937 cell lysates treated with pervanadate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab62350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 72 kDa.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionNon-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen.
    • Tissue specificityWidely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.
    • DomainThe SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins. Some proteins like CLEC1B have a partial ITAM domain (also called hemITAM) containing a single YxxL motif. The interaction with SYK requires CLEC1B homodimerization.
    • Post-translational
      modifications
      Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
      Autophosphorylated. Phosphorylated on tyrosine residues by LYN following receptors engagement. Phosphorylation on Tyr-323 creates a binding site for CBL, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling. Phosphorylation at Tyr-348 creates a binding site for VAV1. Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway (By similarity). Phosphorylation on Ser-297 is very common, it peaks 5 minutes after BCR stimulation, and creates a binding site for YWHAG. Phosphorylation at Tyr-630 creates a binding site for BLNK. Dephosphorylated by PTPN6.
    • Cellular localizationCell membrane. Cytoplasm, cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.10.2 antibody
      • kinase Syk antibody
      • KSYK antibody
      • KSYK_HUMAN antibody
      • p72-Syk antibody
      • p72syk antibody
      • Spleen tyrosine kinase antibody
      • Syk antibody
      • Tyrosine protein kinase SYK antibody
      • Tyrosine-protein kinase SYK antibody
      see all

    Anti-Syk (phospho Y525) antibody [EP575(2)Y] images

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: Syk(pY525) phospho peptide

      Lane 2 sample: Syk non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350) at 1/10000 dilution

      Lane 1 : untreated U937 cell lysates
      Lane 2 : U937 cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 75,80 kDa (why is the actual band size different from the predicted?)
      Beta tubulin shows equal lysate loading amount.

    References for Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350)

    This product has been referenced in:
    • Koo TY  et al. Mycophenolic acid regulates spleen tyrosine kinase to repress tumour necrosis factor-alpha-induced monocyte chemotatic protein-1 production in cultured human aortic endothelial cells. Cell Biol Int 37:19-28 (2013). Human . Read more (PubMed: 23319318) »

    See 1 Publication for this product

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    Thank you for contacting us. The concentration of lot GR28202is 0.214 mg/mL. Please do not hesitate to contact us if you need any more advice or information.

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