Overview

  • Product name
  • Description
    Rabbit polyclonal to Symplekin
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 350 and 400 of Human Symplekin (NP_004810.2).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 165 kDa (predicted molecular weight: 141 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    Symplekin (SYMPK) can associate with tight junctions, functions in the regulation of polyadenylation and promotes gene expression. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation.
  • Cellular localization
    Cytoplasm, cytoskeleton. Cell junction, tight junction; Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells.
  • Database links
  • Alternative names
    • FLJ27092 antibody
    • SPK antibody
    • SYM antibody
    • SYMPK antibody

Anti-Symplekin antibody images

  • All lanes : Anti-Symplekin antibody (ab80274) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 141 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)
  • Detection of Symplekin in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab80274 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References for Anti-Symplekin antibody (ab80274)

This product has been referenced in:
  • Soboleva TA  et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . Read more (PubMed: 28234895) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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