Overview

  • Product nameAnti-Symplekin antibody
    See all Symplekin primary antibodies
  • Description
    Rabbit polyclonal to Symplekin
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1224-1274 of Human Symplekin (NP_004810.2).

  • Positive control
    • Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab99257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is recommended. Antigen retrieval with citrate buffer will enhance staining. Likely to work with frozen sections

Target

  • RelevanceSymplekin (SYMPK) can associate with tight junctions, functions in the regulation of polyadenylation and promotes gene expression. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation.
  • Cellular localizationCytoplasm, cytoskeleton. Cell junction, tight junction; Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells.
  • Database links
  • Alternative names
    • FLJ27092 antibody
    • SPK antibody
    • SYM antibody
    • SYMPK antibody

Anti-Symplekin antibody images

  • ab99257 at 1/250 dilution staining Symplekin in formalin-fixed, paraffin-embedded section of Human colon carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.
  • ab99257 at 1/250 dilution staining Symplekin in formalin-fixed, paraffin-embedded section of Human Seminoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.
  • ab99257 at 1/250 dilution staining Symplekin in formalin-fixed, paraffin-embedded section of Human pancreatic islet cell tumor by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.

References for Anti-Symplekin antibody (ab99257)

ab99257 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99257.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"