Anti-Synapsin I antibody - Synaptic Marker (ab8)

Overview

  • Product nameAnti-Synapsin I antibody - Synaptic Marker
    See all Synapsin I primary antibodies
  • Description
    Rabbit polyclonal to Synapsin I - Synaptic Marker
  • Tested applicationsSuitable for: ICC, ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Cow, Human
  • Immunogen

    Full length native protein (purified) (Cow).

Properties

Applications

Our Abpromise guarantee covers the use of ab8 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 80 kDa.
IP Use at an assay dependent concentration. 1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.
IHC-FoFr Use a concentration of 0.1 µg/ml.
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.

Target

  • FunctionNeuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • Involvement in diseaseDefects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • Sequence similaritiesBelongs to the synapsin family.
  • Post-translational
    modifications
    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

Anti-Synapsin I antibody - Synaptic Marker images

  • Mouse hippocampal primary neurons were fixed, permeabilised and
    labeled with synapsin I antibody ab8 for 1 h at a dilution of 1:500, followed by FITC-conjugated anti rabbit IgG (left panel, green). Co-staining of the same specimen with another antibody against synapsin I (Cy3-conjugated secondary antibody; middle panel, red) confirmed the specificity of both antibodies for the antigen (right panel, yellow colocalisation).

    These images were kindly supplied as part of the review submitted by Christian Specht.

  • ab8 staining Synapsin I in rat Hippocampal neurons from PO/P1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1 % Triton. Samples were incubated with primary antibody, diluted 1/400, for 12 hours at 40C. An AMCA-conjugated donkey polyclonal to rabbit IgG was used at 1/500 dilution as secondary antibody.

    See Abreview

References for Anti-Synapsin I antibody - Synaptic Marker (ab8)

This product has been referenced in:
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . Read more (PubMed: 27662313) »
  • Efthymiou A  et al. Functional screening assays with neurons generated from pluripotent stem cell-derived neural stem cells. J Biomol Screen 19:32-43 (2014). Read more (PubMed: 24019252) »

See all 15 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Human Cultured Cells (neurons derived from iPSCs)
Specification neurons derived from iPSCs
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step Other
Sample Mouse Tissue sections (fixed mouse brain)
Specification fixed mouse brain
Permeabilization Yes - 0.5% TrintonX-100
Fixative Paraformaldehyde
Username

Dr. Hyun Jin Kim

Verified customer

Submitted Aug 20 2013

Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any ad...

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Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you wo...

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Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement....

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the orde...

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Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interest...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cultured Cells (Hippocampal neurons from P0 /P1)
Specification Hippocampal neurons from P0 /P1
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Username

Dr. Senthil Thyagarajan

Verified customer

Submitted Feb 11 2010

Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing...

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I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"