Recombinant
RabMAb

Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

Overview

  • Product name
    Anti-Synapsin I (phospho S553) antibody [E377]
    See all Synapsin I primary antibodies
  • Description
    Rabbit monoclonal [E377] to Synapsin I (phospho S553)
  • Specificity
    This antibody only detects Synapsin phosphorylated on Serine 553
  • Tested applications
    Suitable for: ICC/IF, WB, Dot blotmore details
    Unsuitable for: Flow Cyt,IHC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Ser553 of Synapsin (Human)

  • Positive control
    • Mouse brain lysates, PC-12 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Dot blot 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC or IP.
  • Target

    • Function
      Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
    • Involvement in disease
      Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
    • Sequence similarities
      Belongs to the synapsin family.
    • Post-translational
      modifications
      Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cell junction > synapse. Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Brain protein 4.1 antibody
      • SYN 1 antibody
      • SYN 1a antibody
      • SYN 1b antibody
      • SYN I antibody
      • SYN1 antibody
      • SYN1_HUMAN antibody
      • SYN1a antibody
      • SYN1b antibody
      • Synapsin 1 antibody
      • Synapsin I antibody
      • Synapsin-1 antibody
      • Synapsin1 antibody
      • SynapsinI antibody
      • SYNI antibody
      see all

    Anti-Synapsin I (phospho S553) antibody [E377] images

    • All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

      Lane 1 : Untreated rat brain whole cell lysates
      Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second
    • All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

      Lane 1 : Untreated mouse brain whole cell lysate
      Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution

      Lane 1 : Untreated mouse brain whole cell lysate
      Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer: 2% BSA/TBST

      Dilution buffer: 2% BSA/TBST

    • Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500
    • Immunofluorescent staining of PC-12 cells using ab32532 at a dilution of 1/50 -1/100

    References for Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

    This product has been referenced in:
    • Verstegen AM  et al. Phosphorylation of synapsin I by cyclin-dependent kinase-5 sets the ratio between the resting and recycling pools of synaptic vesicles at hippocampal synapses. J Neurosci 34:7266-80 (2014). Read more (PubMed: 24849359) »
    • Sriram K  et al. Dopaminergic neurotoxicity following pulmonary exposure to manganese-containing welding fumes. Arch Toxicol 84:521-40 (2010). WB ; Rat . Read more (PubMed: 20224926) »

    See all 2 Publications for this product

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